The ELISA Immunoassay:
Grades 9-12
Students will study the specificity of an antibody-antigen reaction by comparing the binding of antibodies and antigens from 4 different types of animals (rabbit, chicken, cow, and horse).  Reagents purchased from Modern Biology Inc., 1-800-733-6544.
Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

Introducing Plasmid DNA into E. coli bacteria:
Grades 10-12
Students will learn about plasmids, and create a strain of bacteria that is resistant to ampicillin. Reagents purchased from Modern Biology Inc., 1-800-733-6544.
Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

DNA Fingerprinting
Grades 10-12
Students will use restriction enzymes and agarose gel electrophoresis to make a DNA fingerprint profile of a number of samples of DNA.  Reagents purchased from Bio-Rad's Biotechnology Explorer kits, 1-800-4BIORAD.
DNA Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

Amplification by the Polymerase Chain Reaction (PCR).
Grades 10-12
Students will use the powerful PCR technique to amplify a segment of DNA.
Reagents purchased from Carolina Biological Supply Company, 1-800-334-5551.
Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

Tobacco  Mosaic Virus Infection
Grades 7-12
Students will infect bean plants will tobacco mosaic virus.
Reagents purchased from Carolina Biological Supply Company, 1-800-334-5551.
Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.
 

Drospohila Polytene Chromosomes
Grades 9-12
Students will prepare slides of the giant polytene chromosomes from salivary glands of the fruit fly drosophila.
Reagents purchased from Carolina Biological Supply Company, 1-800-334-5551.
Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

Preparation of Human Chromosome Spreads:
Grades 9-12
Students will prepare and view slides of human chromosomes from cancer cells.
Reagents purchased from CellServ, 202-319-5725.
Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

pGLO Transformation:
Grades 9-12
Students will transfer the plasmid pGLO into bacterial cells, causing the bacteria to glow under an ultraviolet light source. Reagents purchased from Bio-Rad's Biotechnology Explorer kits, 1-800-4BIORAD.
DNA Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.

Green Fluorescent Protein (GFP) Purification:
Grades 10-12
Students will isolate GFP from a culture of bacteria, using a chromatography procedure.
Reagents purchased from Bio-Rad's Biotechnology Explorer kits, 1-800-4BIORAD.
DNA Contact for more information:  Dina Markowitz, Ph.D., University of Rochester, (716)275-8056.